Gene Set Enrichment Analysis (GSEA) is a freely available tool developed at the Broad Institute that allows analysis of groups of genes in the context of gene expression experiments. The groups of genes under investigation can include thousands of functionally associated, positional or other types of sets annotated in the the MsigDb collections or custom gene sets relevant to an experiment. In this IAP, the rationale for GSEA will be reviewed and hands-on instruction in input data setup, execution of analyses and assembly and interpretation of results will be provided. Single-sample GSEA variant will be demonstrated and the application of GSEA to different data types will be discussed. Example datasets will be provided but attendees are encouraged to bring their own data.

Eligibility: MIT researchers affiliated with the Koch Institute, the Biology Department, the Center for Environmental Health Sciences or the Department of Biological Engineering.

Registration is required.